Poplar trees for phytoremediation of high levels of nitrate and applications in bioenergy.
Identifieur interne : 001718 ( Main/Exploration ); précédent : 001717; suivant : 001719Poplar trees for phytoremediation of high levels of nitrate and applications in bioenergy.
Auteurs : Vanessa Castro-Rodríguez [Espagne] ; Angel García-Gutiérrez [Espagne] ; Javier Canales [Espagne] ; Rafael A. Ca As [Espagne] ; Edward G. Kirby [États-Unis] ; Concepci N Avila [Espagne] ; Francisco M. Cánovas [Espagne]Source :
- Plant biotechnology journal [ 1467-7652 ] ; 2016.
Descripteurs français
- KwdFr :
- ARN messager (génétique), ARN messager (métabolisme), Analyse de profil d'expression de gènes (MeSH), Arbres (croissance et développement), Arbres (effets des médicaments et des substances chimiques), Arbres (génétique), Arbres (métabolisme), Azote (métabolisme), Azote (pharmacologie), Biocarburants (MeSH), Biomasse (MeSH), Carbone (métabolisme), Chlorophylle (métabolisme), Dépollution biologique de l'environnement (effets des médicaments et des substances chimiques), Glutamate-ammonia ligase (métabolisme), Lignine (métabolisme), Métabolisme glucidique (effets des médicaments et des substances chimiques), Métabolisme glucidique (génétique), Nitrates (métabolisme), Phénotype (MeSH), Populus (croissance et développement), Populus (effets des médicaments et des substances chimiques), Populus (génétique), Populus (métabolisme), Protéines végétales (génétique), Protéines végétales (métabolisme), Reproductibilité des résultats (MeSH), Régulation de l'expression des gènes végétaux (effets des médicaments et des substances chimiques), Solubilité (MeSH), Transcriptome (génétique), Végétaux génétiquement modifiés (MeSH).
- MESH :
- croissance et développement : Arbres, Populus.
- effets des médicaments et des substances chimiques : Arbres, Dépollution biologique de l'environnement, Métabolisme glucidique, Populus, Régulation de l'expression des gènes végétaux.
- génétique : ARN messager, Arbres, Métabolisme glucidique, Populus, Protéines végétales, Transcriptome.
- métabolisme : ARN messager, Arbres, Azote, Carbone, Chlorophylle, Glutamate-ammonia ligase, Lignine, Nitrates, Populus, Protéines végétales.
- pharmacologie : Azote.
- Analyse de profil d'expression de gènes, Biocarburants, Biomasse, Phénotype, Reproductibilité des résultats, Solubilité, Végétaux génétiquement modifiés.
English descriptors
- KwdEn :
- Biodegradation, Environmental (drug effects), Biofuels (MeSH), Biomass (MeSH), Carbohydrate Metabolism (drug effects), Carbohydrate Metabolism (genetics), Carbon (metabolism), Chlorophyll (metabolism), Gene Expression Profiling (MeSH), Gene Expression Regulation, Plant (drug effects), Glutamate-Ammonia Ligase (metabolism), Lignin (metabolism), Nitrates (metabolism), Nitrogen (metabolism), Nitrogen (pharmacology), Phenotype (MeSH), Plant Proteins (genetics), Plant Proteins (metabolism), Plants, Genetically Modified (MeSH), Populus (drug effects), Populus (genetics), Populus (growth & development), Populus (metabolism), RNA, Messenger (genetics), RNA, Messenger (metabolism), Reproducibility of Results (MeSH), Solubility (MeSH), Transcriptome (genetics), Trees (drug effects), Trees (genetics), Trees (growth & development), Trees (metabolism).
- MESH :
- chemical , genetics : Plant Proteins, RNA, Messenger.
- chemical , metabolism : Carbon, Chlorophyll, Glutamate-Ammonia Ligase, Lignin, Nitrates, Nitrogen, Plant Proteins, RNA, Messenger.
- chemical , pharmacology : Nitrogen.
- chemical : Biofuels.
- drug effects : Biodegradation, Environmental, Carbohydrate Metabolism, Gene Expression Regulation, Plant, Populus, Trees.
- genetics : Carbohydrate Metabolism, Populus, Transcriptome, Trees.
- growth & development : Populus, Trees.
- metabolism : Populus, Trees.
- Biomass, Gene Expression Profiling, Phenotype, Plants, Genetically Modified, Reproducibility of Results, Solubility.
Abstract
The utilization of high amounts of nitrate fertilizers for crop yield leads to nitrate pollution of ground and surface waters. In this study, we report the assimilation and utilization of nitrate luxuriant levels, 20 times more than the highest N fertilizer application in Europe, by transgenic poplars overexpressing a cytosolic glutamine synthetase (GS1). In comparison with the wild-type controls, transgenic plants grown under high N levels exhibited increased biomass (171.6%) and accumulated higher levels of proteins, chlorophylls and total sugars such as glucose, fructose and sucrose. These plants also exhibited greater nitrogen-use efficiency particularly in young leaves, suggesting that they are able to translocate most of the resources to the above-ground part of the plant to produce biomass. The transgenic poplar transcriptome was greatly affected in response to N availability with 1237 genes differentially regulated in high N, while only 632 genes were differentially expressed in untransformed plants. Many of these genes are essential in the adaptation and response against N excess and include those involved in photosynthesis, cell wall formation and phenylpropanoid biosynthesis. Cellulose production in the transgenic plants was fivefold higher than in control plants, indicating that transgenic poplars represent a potential feedstock for applications in bioenergy. In conclusion, our results show that GS transgenic poplars can be used not only for improving growth and biomass production but also as an important resource for potential phytoremediation of nitrate pollution.
DOI: 10.1111/pbi.12384
PubMed: 25923308
Affiliations:
- Espagne, États-Unis
- Andalousie, New Jersey
- Malaga, New Brunswick (New Jersey)
- Université Rutgers, Université de Malaga
Links toward previous steps (curation, corpus...)
Le document en format XML
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Cánovas</name><affiliation wicri:level="4"><nlm:affiliation>Departamento de Biología Molecular y Bioquímica, Instituto Andaluz de Biotecnología, Universidad de Málaga, Málaga, Spain.</nlm:affiliation><country xml:lang="fr">Espagne</country><wicri:regionArea>Departamento de Biología Molecular y Bioquímica, Instituto Andaluz de Biotecnología, Universidad de Málaga, Málaga</wicri:regionArea><orgName type="university">Université de Malaga</orgName><placeName><settlement type="city">Malaga</settlement><region nuts="2" type="region">Andalousie</region></placeName></affiliation></author></analytic><series><title level="j">Plant biotechnology journal</title><idno type="eISSN">1467-7652</idno><imprint><date when="2016" type="published">2016</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Biodegradation, Environmental (drug effects)</term><term>Biofuels (MeSH)</term><term>Biomass (MeSH)</term><term>Carbohydrate Metabolism (drug effects)</term><term>Carbohydrate Metabolism (genetics)</term><term>Carbon (metabolism)</term><term>Chlorophyll (metabolism)</term><term>Gene Expression Profiling (MeSH)</term><term>Gene Expression Regulation, Plant (drug effects)</term><term>Glutamate-Ammonia Ligase (metabolism)</term><term>Lignin (metabolism)</term><term>Nitrates (metabolism)</term><term>Nitrogen (metabolism)</term><term>Nitrogen (pharmacology)</term><term>Phenotype (MeSH)</term><term>Plant Proteins (genetics)</term><term>Plant Proteins (metabolism)</term><term>Plants, Genetically Modified (MeSH)</term><term>Populus (drug effects)</term><term>Populus (genetics)</term><term>Populus (growth & development)</term><term>Populus (metabolism)</term><term>RNA, Messenger (genetics)</term><term>RNA, Messenger (metabolism)</term><term>Reproducibility of Results (MeSH)</term><term>Solubility (MeSH)</term><term>Transcriptome (genetics)</term><term>Trees (drug effects)</term><term>Trees (genetics)</term><term>Trees (growth & development)</term><term>Trees (metabolism)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>ARN messager (génétique)</term><term>ARN messager (métabolisme)</term><term>Analyse de profil d'expression de gènes (MeSH)</term><term>Arbres (croissance et développement)</term><term>Arbres (effets des médicaments et des substances chimiques)</term><term>Arbres (génétique)</term><term>Arbres (métabolisme)</term><term>Azote (métabolisme)</term><term>Azote (pharmacologie)</term><term>Biocarburants (MeSH)</term><term>Biomasse (MeSH)</term><term>Carbone (métabolisme)</term><term>Chlorophylle (métabolisme)</term><term>Dépollution biologique de l'environnement (effets des médicaments et des substances chimiques)</term><term>Glutamate-ammonia ligase (métabolisme)</term><term>Lignine (métabolisme)</term><term>Métabolisme glucidique (effets des médicaments et des substances chimiques)</term><term>Métabolisme glucidique (génétique)</term><term>Nitrates (métabolisme)</term><term>Phénotype (MeSH)</term><term>Populus (croissance et développement)</term><term>Populus (effets des médicaments et des substances chimiques)</term><term>Populus (génétique)</term><term>Populus (métabolisme)</term><term>Protéines végétales (génétique)</term><term>Protéines végétales (métabolisme)</term><term>Reproductibilité des résultats (MeSH)</term><term>Régulation de l'expression des gènes végétaux (effets des médicaments et des substances chimiques)</term><term>Solubilité (MeSH)</term><term>Transcriptome (génétique)</term><term>Végétaux génétiquement modifiés (MeSH)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Plant Proteins</term><term>RNA, Messenger</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Carbon</term><term>Chlorophyll</term><term>Glutamate-Ammonia Ligase</term><term>Lignin</term><term>Nitrates</term><term>Nitrogen</term><term>Plant Proteins</term><term>RNA, Messenger</term></keywords><keywords scheme="MESH" type="chemical" qualifier="pharmacology" xml:lang="en"><term>Nitrogen</term></keywords><keywords scheme="MESH" type="chemical" xml:lang="en"><term>Biofuels</term></keywords><keywords scheme="MESH" qualifier="croissance et développement" xml:lang="fr"><term>Arbres</term><term>Populus</term></keywords><keywords scheme="MESH" qualifier="drug effects" xml:lang="en"><term>Biodegradation, Environmental</term><term>Carbohydrate Metabolism</term><term>Gene Expression Regulation, Plant</term><term>Populus</term><term>Trees</term></keywords><keywords scheme="MESH" qualifier="effets des médicaments et des substances chimiques" xml:lang="fr"><term>Arbres</term><term>Dépollution biologique de l'environnement</term><term>Métabolisme glucidique</term><term>Populus</term><term>Régulation de l'expression des gènes végétaux</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Carbohydrate Metabolism</term><term>Populus</term><term>Transcriptome</term><term>Trees</term></keywords><keywords scheme="MESH" qualifier="growth & development" xml:lang="en"><term>Populus</term><term>Trees</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>ARN messager</term><term>Arbres</term><term>Métabolisme glucidique</term><term>Populus</term><term>Protéines végétales</term><term>Transcriptome</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Populus</term><term>Trees</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>ARN messager</term><term>Arbres</term><term>Azote</term><term>Carbone</term><term>Chlorophylle</term><term>Glutamate-ammonia ligase</term><term>Lignine</term><term>Nitrates</term><term>Populus</term><term>Protéines végétales</term></keywords><keywords scheme="MESH" qualifier="pharmacologie" xml:lang="fr"><term>Azote</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Biomass</term><term>Gene Expression Profiling</term><term>Phenotype</term><term>Plants, Genetically Modified</term><term>Reproducibility of Results</term><term>Solubility</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Analyse de profil d'expression de gènes</term><term>Biocarburants</term><term>Biomasse</term><term>Phénotype</term><term>Reproductibilité des résultats</term><term>Solubilité</term><term>Végétaux génétiquement modifiés</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The utilization of high amounts of nitrate fertilizers for crop yield leads to nitrate pollution of ground and surface waters. In this study, we report the assimilation and utilization of nitrate luxuriant levels, 20 times more than the highest N fertilizer application in Europe, by transgenic poplars overexpressing a cytosolic glutamine synthetase (GS1). In comparison with the wild-type controls, transgenic plants grown under high N levels exhibited increased biomass (171.6%) and accumulated higher levels of proteins, chlorophylls and total sugars such as glucose, fructose and sucrose. These plants also exhibited greater nitrogen-use efficiency particularly in young leaves, suggesting that they are able to translocate most of the resources to the above-ground part of the plant to produce biomass. The transgenic poplar transcriptome was greatly affected in response to N availability with 1237 genes differentially regulated in high N, while only 632 genes were differentially expressed in untransformed plants. Many of these genes are essential in the adaptation and response against N excess and include those involved in photosynthesis, cell wall formation and phenylpropanoid biosynthesis. Cellulose production in the transgenic plants was fivefold higher than in control plants, indicating that transgenic poplars represent a potential feedstock for applications in bioenergy. In conclusion, our results show that GS transgenic poplars can be used not only for improving growth and biomass production but also as an important resource for potential phytoremediation of nitrate pollution. </div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">25923308</PMID><DateCompleted><Year>2016</Year><Month>12</Month><Day>13</Day></DateCompleted><DateRevised><Year>2016</Year><Month>12</Month><Day>30</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1467-7652</ISSN><JournalIssue CitedMedium="Internet"><Volume>14</Volume><Issue>1</Issue><PubDate><Year>2016</Year><Month>Jan</Month></PubDate></JournalIssue><Title>Plant biotechnology journal</Title><ISOAbbreviation>Plant Biotechnol J</ISOAbbreviation></Journal><ArticleTitle>Poplar trees for phytoremediation of high levels of nitrate and applications in bioenergy.</ArticleTitle><Pagination><MedlinePgn>299-312</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1111/pbi.12384</ELocationID><Abstract><AbstractText>The utilization of high amounts of nitrate fertilizers for crop yield leads to nitrate pollution of ground and surface waters. In this study, we report the assimilation and utilization of nitrate luxuriant levels, 20 times more than the highest N fertilizer application in Europe, by transgenic poplars overexpressing a cytosolic glutamine synthetase (GS1). In comparison with the wild-type controls, transgenic plants grown under high N levels exhibited increased biomass (171.6%) and accumulated higher levels of proteins, chlorophylls and total sugars such as glucose, fructose and sucrose. These plants also exhibited greater nitrogen-use efficiency particularly in young leaves, suggesting that they are able to translocate most of the resources to the above-ground part of the plant to produce biomass. The transgenic poplar transcriptome was greatly affected in response to N availability with 1237 genes differentially regulated in high N, while only 632 genes were differentially expressed in untransformed plants. Many of these genes are essential in the adaptation and response against N excess and include those involved in photosynthesis, cell wall formation and phenylpropanoid biosynthesis. Cellulose production in the transgenic plants was fivefold higher than in control plants, indicating that transgenic poplars represent a potential feedstock for applications in bioenergy. In conclusion, our results show that GS transgenic poplars can be used not only for improving growth and biomass production but also as an important resource for potential phytoremediation of nitrate pollution. </AbstractText><CopyrightInformation>© 2015 Society for Experimental Biology, Association of Applied Biologists and John Wiley & Sons Ltd.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Castro-Rodríguez</LastName><ForeName>Vanessa</ForeName><Initials>V</Initials><AffiliationInfo><Affiliation>Departamento de Biología Molecular y Bioquímica, Instituto Andaluz de Biotecnología, Universidad de Málaga, Málaga, Spain.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>García-Gutiérrez</LastName><ForeName>Angel</ForeName><Initials>A</Initials><AffiliationInfo><Affiliation>Departamento de Biología Molecular y Bioquímica, Instituto Andaluz de Biotecnología, Universidad de Málaga, Málaga, Spain.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Canales</LastName><ForeName>Javier</ForeName><Initials>J</Initials><AffiliationInfo><Affiliation>Departamento de Biología Molecular y Bioquímica, Instituto Andaluz de Biotecnología, Universidad de Málaga, Málaga, Spain.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Cañas</LastName><ForeName>Rafael A</ForeName><Initials>RA</Initials><Identifier Source="ORCID">http://orcid.org/0000-0001-9727-5585</Identifier><AffiliationInfo><Affiliation>Departamento de Biología Molecular y Bioquímica, Instituto Andaluz de Biotecnología, Universidad de Málaga, Málaga, Spain.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Kirby</LastName><ForeName>Edward G</ForeName><Initials>EG</Initials><AffiliationInfo><Affiliation>Department of Biological Sciences, Rutgers University, Newark, NJ, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Avila</LastName><ForeName>Concepción</ForeName><Initials>C</Initials><AffiliationInfo><Affiliation>Departamento de Biología Molecular y Bioquímica, Instituto Andaluz de Biotecnología, Universidad de Málaga, Málaga, Spain.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Cánovas</LastName><ForeName>Francisco M</ForeName><Initials>FM</Initials><AffiliationInfo><Affiliation>Departamento de Biología Molecular y Bioquímica, Instituto Andaluz de Biotecnología, Universidad de Málaga, Málaga, Spain.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2015</Year><Month>04</Month><Day>28</Day></ArticleDate></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>Plant Biotechnol J</MedlineTA><NlmUniqueID>101201889</NlmUniqueID><ISSNLinking>1467-7644</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D056804">Biofuels</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D009566">Nitrates</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D010940">Plant Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D012333">RNA, Messenger</NameOfSubstance></Chemical><Chemical><RegistryNumber>1406-65-1</RegistryNumber><NameOfSubstance UI="D002734">Chlorophyll</NameOfSubstance></Chemical><Chemical><RegistryNumber>7440-44-0</RegistryNumber><NameOfSubstance UI="D002244">Carbon</NameOfSubstance></Chemical><Chemical><RegistryNumber>9005-53-2</RegistryNumber><NameOfSubstance UI="D008031">Lignin</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 6.3.1.2</RegistryNumber><NameOfSubstance UI="D005974">Glutamate-Ammonia Ligase</NameOfSubstance></Chemical><Chemical><RegistryNumber>N762921K75</RegistryNumber><NameOfSubstance UI="D009584">Nitrogen</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D001673" MajorTopicYN="N">Biodegradation, Environmental</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="N">drug effects</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D056804" MajorTopicYN="Y">Biofuels</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D018533" MajorTopicYN="N">Biomass</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D050260" MajorTopicYN="N">Carbohydrate Metabolism</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="N">drug effects</QualifierName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D002244" MajorTopicYN="N">Carbon</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D002734" MajorTopicYN="N">Chlorophyll</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D020869" MajorTopicYN="N">Gene Expression Profiling</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D018506" MajorTopicYN="N">Gene Expression Regulation, Plant</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="N">drug effects</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D005974" MajorTopicYN="N">Glutamate-Ammonia Ligase</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D008031" MajorTopicYN="N">Lignin</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D009566" MajorTopicYN="N">Nitrates</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D009584" MajorTopicYN="N">Nitrogen</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName><QualifierName UI="Q000494" MajorTopicYN="N">pharmacology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010641" MajorTopicYN="N">Phenotype</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D010940" MajorTopicYN="N">Plant Proteins</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D030821" MajorTopicYN="N">Plants, Genetically Modified</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D032107" MajorTopicYN="N">Populus</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="N">drug effects</QualifierName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000254" MajorTopicYN="N">growth & development</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D012333" MajorTopicYN="N">RNA, Messenger</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D015203" MajorTopicYN="N">Reproducibility of Results</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D012995" MajorTopicYN="N">Solubility</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D059467" MajorTopicYN="N">Transcriptome</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D014197" MajorTopicYN="N">Trees</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="N">drug effects</QualifierName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000254" MajorTopicYN="N">growth & development</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading></MeshHeadingList><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">Populus</Keyword><Keyword MajorTopicYN="N">bioenergy</Keyword><Keyword MajorTopicYN="N">biomass</Keyword><Keyword MajorTopicYN="N">glutamine synthetase</Keyword><Keyword MajorTopicYN="N">nitrate pollution</Keyword><Keyword MajorTopicYN="N">transgenic trees</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2014</Year><Month>12</Month><Day>12</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2015</Year><Month>03</Month><Day>19</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2015</Year><Month>03</Month><Day>20</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2015</Year><Month>4</Month><Day>30</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2015</Year><Month>4</Month><Day>30</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2016</Year><Month>12</Month><Day>15</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">25923308</ArticleId><ArticleId IdType="doi">10.1111/pbi.12384</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Espagne</li><li>États-Unis</li></country><region><li>Andalousie</li><li>New Jersey</li></region><settlement><li>Malaga</li><li>New Brunswick (New Jersey)</li></settlement><orgName><li>Université Rutgers</li><li>Université de Malaga</li></orgName></list><tree><country name="Espagne"><region name="Andalousie"><name sortKey="Castro Rodriguez, Vanessa" sort="Castro Rodriguez, Vanessa" uniqKey="Castro Rodriguez V" first="Vanessa" last="Castro-Rodríguez">Vanessa Castro-Rodríguez</name></region><name sortKey="Avila, Concepci N" sort="Avila, Concepci N" uniqKey="Avila C" first="Concepci N" last="Avila">Concepci N Avila</name><name sortKey="Ca As, Rafael A" sort="Ca As, Rafael A" uniqKey="Ca As R" first="Rafael A" last="Ca As">Rafael A. Ca As</name><name sortKey="Canales, Javier" sort="Canales, Javier" uniqKey="Canales J" first="Javier" last="Canales">Javier Canales</name><name sortKey="Canovas, Francisco M" sort="Canovas, Francisco M" uniqKey="Canovas F" first="Francisco M" last="Cánovas">Francisco M. Cánovas</name><name sortKey="Garcia Gutierrez, Angel" sort="Garcia Gutierrez, Angel" uniqKey="Garcia Gutierrez A" first="Angel" last="García-Gutiérrez">Angel García-Gutiérrez</name></country><country name="États-Unis"><region name="New Jersey"><name sortKey="Kirby, Edward G" sort="Kirby, Edward G" uniqKey="Kirby E" first="Edward G" last="Kirby">Edward G. Kirby</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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